Research Reagent for the Determination of Reticulated Platelets and Absolute Count Determination of Thrombocytes
A. Sample Preparation
1. Addition of CD42b PE
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2. Addition of EDTA whole blood
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3. RNA Staining
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B. Measurement
set FSC and SSC amlifier gains to log mode. Region 1 set on CD42b positive thrombocytes, region set on counting beads
C. Data Evaluation
(1)Calculation absolute counts of thrombocytes: Determine the number of thrombocytes in region 1 and the number of counting beads in region 2.
(2)Determining the percentage of reticulated platelets: after setting a logical gate on CD42b positive thrombocytes, the percentage of reticulated platelets is determined in region 3 in dot plot diagram SSC vs. FL1
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