Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Bloods
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A. Sample Preparation
for low and high stimulant. Simple as you know it from immunofluorescence analysis:
1. Activation  | 2. Oxidation  |
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3. Lysing and Fixation  | 4. Washing and DNA Staining  |
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B. Measurement
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Gating on leukocytes after DNA staining: Discrimination of bacteria, single leukocytes and doublets.
C. Data Evaluation
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Gate on granulocytes and determine the percentage of oxidizing cells and their enzymatic activity (= mean fluorescence = number of cleaved substrate).