Clinical Diagnostic for the Determination of Allergen Induced Basophil Degranulation
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A. Sample Preparation
1. Cell Isolation  | 2. Cell Culture  |
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3. Cell Harvest  | 4. Cell Staining  |
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B. Measurement
acquire data by setting the trigger threshold on fluorecence 2.
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C. Data Evaluation
Gate on basophiles expressing high amounts of IgE during data analysis (log FL2/in SCC)
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Determine the percentage of activativity (gp53 positive) cells:
1. Unstimulated control
2. Stimulation with fMLP
3. Stimulation with grass pollen mixture
